National Human Neural Stem Cell Resource provides neural stem cells
harvested from the post-natal, post-mortem, human brain or derived from
induced pluripotent stem cells to the research community for stem cell
research. Several brain areas as well as cultures from normal and
genetically mutant specimens are represented in the Resource.
cell research will increase our understanding of the nervous
system and may allow us to develop treatments for currently
incurable brain diseases and injuries.
Resource encourages stem cell research for the study of
these neural stem cells as potential transplantable tissue
for the repair of injury such as that sustained during
traumatic brain injury or stroke, for the repair of pathological
processes such as those seen in the neurogenetic diseases
glutaric acidemia (Type I) or Leigh's disease, or for repair
of neurodegenerative processes such as those seen in Parkinson's
or Alzheimer Diseases.
In addition, the stem cells should be used for stem cell research aimed
at the detailed study of mechanisms of neural differentiation and
transdifferentiation, the genetic and environmental signals that direct
the specialization of the cells into particular cell types, and as
invitro models of diseases, such as autism and Fragile X syndrome.
Philip H. Schwartz, Ph.D., Director
A primary culture of proliferating cells showed a high proportion
of nestin (red) to GFAP (blue) staining in monolayer culture;
co-staining was relatively frequent (10x).
B) Budding neurosphere showed nestin cells (red) at the
circumference with a much higher proportion of GFAP (blue)
to nestin in the interior of the sphere (10x).
C) Nestin (green) and Sox2 (red) staining in proliferating
hNPCs (40x). Arrows show occasional nestin-negative/Sox2-positive
D) Doublecortin (DCX) staining of hNPCs revealed a subpopulation
of small, 5 - 10 um diameter, DCX-positive (green) cells
against a phase-contrast background (40x).
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