Methods Development

 

 

Table of Contents

 

Section I. Laboratory Essentials

1. The Stem Cell Laboratory: Design, Equipment, and Oversight
Robin L. Wesselschmidt and Philip H. Schwartz

2. Stem Cell Banks: Preserving Cell Lines, Maintaining Genetic Integrity, and Advancing Research
Lesley Young, Lyn Healy, and Glyn N. Stacey

 

Section II. Derivation

3. Embryonic Stem Cell Derivation from Human Embryos
Paul Lerou

 4. Derivation of Human Parthenogenetic Stem Cell Lines
Nikolay Turovets, Andrey Semechkin, Leonid Kuzmichev, Jeffrey Janus, Larissa Agapova, and Elena Revazova

 5. Generation of Induced Pluripotent Stem Cell Lines from Human Fibroblasts via Retroviral Gene Transfer
Justine D. Miller and Thorsten M. Schlaeger

 6. Derivation of Induced Pluripotent Stem Cells by Lentiviral Transduction
Hubert E. Nethercott, David J. Brick, and Philip H. Schwartz

7. Transgene-free Production of Pluripotent Stem Cells using PiggyBac Transposons
Knut Woltjen, Riikka Hämäläinen, Mark Kibschull, Maria Mileikovsky, and Andras Nagy

 

Section III. Growth, Maintenance and Expansion

 8. Traditional Human Embryonic Stem Cell Culture
Philip H. Schwartz, David J. Brick, Hubert E. Nethercott, and Alexander E. Stover

 9. Xeno-Free Culture of Human Pluripotent Stem Cells
Rosita Bergström, Susanne Ström, Frida Holm, Anis Feki, and Outi Hovatta

 10. Adaptation of Human Pluripotent Stem Cells to Feeder-Free Conditions in Chemically-Defined Medium with Enzymatic Single-Cell Passaging
Alexander E. Stover and Philip H. Schwartz

 11. GMP Scale-Up and Banking of Pluripotent Stem Cells for Cellular Therapy Applications
Lara J. Ausubel, Patricia M. Lopez, and Larry A. Couture

12. Culture of Human Pluripotent Stem Cells on Glass Slides for High-Resolution Imaging
Victoria Fox

 

Section IV. Characterization

13. Classical Cytogenetics: Karyotyping Techniques
Steven E. Bates

14. FISH Analysis of Human Pluripotent Stem Cells
Suzanne E. Peterson, Jerold Chun, and Jeanne Loring

 15. Immunocyhemical Analysis of Human Pluripotent Stem Cells
Hubert E. Nethercott, David J. Brick, and Philip H. Schwartz

16. Flow Cytometric Analysis of Human Pluripotent Stem Cells
Mirabelle SH. Ho, Andrew Fryga, and Andrew L. Laslett

 17. The Teratoma Assay: An In vivo Assessment of Pluripotency
Robin L. Wesselschmidt

 18. Detection of Copy Number Variation using SNP Genotyping
Gregory M. Cooper and Heather C. Mefford

 19. Genome-wide Epigenetic Analysis of Human Pluripotent Stem Cells by ChIP and ChIP-Seq
Michael J. Hitchler and Judd C. Rice

 20. Basic Approaches to Gene Expression Analysis of Stem Cells by Microarrays
Bernhard Schuldt, Qiong Lin, Franz-Josef Müller, and Jeanne F. Loring

 21. Development of High Content Screening Approaches and Analysis for Human Pluripotent Stem Cells
Sean P. Sherman, Jackelyn A. Alva, Kaushali Thakore-Shah, and April D. Pyle

 22. Quantitative Proteome and Phosphoproteome Analysis of Human Pluripotent Stem Cells
Javier Muñoz and Albert J. R. Heck

 

Section V. Genetic Manipulation (Gene Modulation)

 23. Lentivirus-mediated Modification of Pluripotent Stem Cells
Ruchi Bajpai

 24. Nucleofection of Human Embryonic Stem Cells
Helen Fong, K. A. Hohenstein Elliott, Leslie F. Lock, and Peter J. Donovan

25. Non-viral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells
Sujoy K. Dhara, Anirban Majumder, Mahesh C. Dodla, and Steven L. Stice

 26. Gene Targeting in Human Pluripotent Stem Cells
Ying Liu and Mahendra Rao

27. Episomal Transgene Expression in Pluripotent Stem Cells
Michele M.P. Lufino, Anna R. Popplestone, Sally A. Cowley, Pauline A.H. Edser, William S. James, and Richard Wade-Martins

 

Section VI: Differentiation

28. The Generation of Embryoid Bodies from Feeder-based or Feeder-free Human Pluripotent Stem Cell Cultures
Alexander E. Stover and Philip H. Schwartz

 29. Derivation of Oligodendrocyte Progenitor Cells from Human Embryonic Stem Cells
Jason Sharp, Maya Hatch, Gabriel Nistor, and Hans Keirstead

 30. Directed Differentiation of Dopamine Neurons From Human Pluripotent Stem Cells
Lixiang Ma, Yan Liu, and Su-Chun Zhang

 31. Methods for the Derivation and Use of Cardiomyocytes from Human Pluripotent Stem Cells
Wei-Zhong Zhu, Benjamin Van Biber, and Michael A. Laflamme

 32. In Vivo Evaluation of Putative Hematopoietic Stem Cells Derived from Human Pluripotent Stem Cells
Melinda K. Hexum, Xinghui Tian, and Dan S. Kaufman

 33. Differentiation of Dendritic Cells from Human Embryonic Stem Cells
Kathryn M. Silk, Su-Yi Tseng, Kevin P. Nishimoto, Jane S. Lebkowski, Anita Reddy, and Paul J. Fairchild

 

 

 

Other Works by Dr. Schwartz in Methods Development