This NIH-sponsored stem cell training course will provide hands-on training for investigators to learn how to culture, manipulate, and differentiate embryonic stem cells from humans in vitro. This course brings together some of the leading experts on embryonic stem cell technology, and through comparative approaches, trains students in the successful culture, maintenance, and manipulation of embryonic stem cells.
The course is unique not only because of its diverse and highly skilled faculty, but also because it provides students with experience with both embryonic and neural stem cells.
This cross-training approach will give the students both depth and breadth of understanding of stem cell biology that will help them in the design of their future research projects.
The overall goal of the course is to send students back to their labs with the ability to efficiently set up and conduct human embryonic stem cell research.
Each course will cover
- Demonstration of proper growth conditions for cells
- Proper freeze-thaw cycling and preparation of cell passages
- Use of co-culture techniques
- Use of cell separation procedures
- Review of basic good laboratory practices for use of human biological materials
- Development of protocols that support the characterization of embryonic stem cells
- Application of standard research protocols for directed differentiation of embryonic stem cells
- Employment of standard detection methods for infectious organisms or other contaminants
- Cross-training of techniques used in other applications of stem cell biology
Each ten-day course will be limited to twelve participants who will be chosen from throughout the US, based on their potential to perform outstanding research in this field.
The next course will be held March 7-16,2006 in southern
California at CHOC Research Institute in Orange, California