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Due to time constraints, the human embryonic stem cell culture training course will not be taught in strict temporal synchrony with respect to the natural history of stem cells as they progress from thawing through expansion to differentiation.
At 4:00pm each day, the participants and faculty will meet in the lecture room to review the days activities and to solicit points for discussion at the next morning's breakfast. This will be done in oral and written formats. In addition, at the 4:00pm session, a pertinent manuscript will be handed out for discussion, in the form of a journal club, during the next day's lunch.
Free time is built into each day and an extended free time period is provided from lunch Saturday to lunch Sunday. In addition, all dinners will be casual in nature, with no formal scientific content, but they will encourage discussion and foster relationships for possible future collaborations and/or sharing of ideas/techniques/troubleshooting, etc.
The aims of the lectures are divided into the following categories (with responsible faculty given in parentheses). These aims are highlighted in the following table.
A. Culture techniques (Schwartz, Loring, Klassen)
B. Derivation of ES cells and neural stem cells (Loring,
Schwartz, Snyder)
C. Maintenance of human ES cells (Loring, Carpenter)
D. Immunocytochemistry and confocal microscopy (Schwartz, Klassen, Palmer)
E. Differentiation (Carpenter, Duncan, Schwartz)
F. Characterization (Schwartz, Carpenter, Duncan, Klassen, Palmer)
G. NIH funding and policies (Chiu)
|
Course Day |
Time |
Activity |
Faculty |
|
Monday |
Arrival and Check-in |
|
|
|
Tuesday |
8:00am |
Introductions/Breakfast |
Schwartz
Klassen |
| |
9:00am |
Lecture: Safety Training; Infection Control/Monitoring
Lab: Facilities Tour
Culture techniques |
Schwartz
Klassen
|
| |
12:00pm |
Lunch |
Schwartz
Klassen |
| |
1:30pm |
Lecture: Microscopy; Differentiation I
Lab: Differentiation of hESCs/hNSCs
Culture techniques & Differentiation |
Schwartz
Klassen
|
| |
4:00pm |
Recap/Evaluation |
Schwartz
Klassen
|
| |
5:00pm |
Free Time |
-------- |
| |
7:00pm |
Dinner
Introduction of Dr. Loring |
Schwartz
Klassen
Loring |
| |
|
|
|
|
Wednesday |
8:00am |
Review/Breakfast |
Schwartz
Klassen
Loring |
| |
9:00am |
Lecture: Media; Feeder Layers; Substrates
Lab: Media; Feeder Layers; Substrates
Derivation of ES cells and neural stem cells & Maintenance of human ES cells
|
Schwartz
Klassen
Loring |
| |
12:00pm |
Lunch/Journal Club |
Schwartz
Klassen
Loring |
| |
1:30pm |
Lecture: Thawing/Plating Cells
Lab: Thawing/Plating Cells
Derivation of ES cells and neural stem cells & Maintenance of human ES cells |
Schwartz
Klassen
Loring |
| |
4:00pm |
Recap/Evaluation |
Schwartz
Klassen
Loring |
| |
5:00pm |
Free Time |
-------- |
| |
7:00pm |
Dinner
Introduction of Dr. Palmer |
Schwartz
Klassen
Palmer |
| |
|
|
|
|
Thursday |
8:00am |
Review/Breakfast |
Schwartz
Klassen
Palmer |
| |
9:00am |
Lecture: Immunocytochemistry; Fluorescence/Confocal Microscopy
Immunocytochemistry and confocal microscopy |
Schwartz
Klassen
Palmer |
| |
12:00pm |
Lunch/Journal Club |
Schwartz
Klassen
Palmer |
| |
1:30pm |
Lab: Immunocytochemistry; Fluorescence/Confocal Microscopy
Immunocytochemistry and confocal microscopy |
Schwartz
Klassen
Palmer |
| |
4:00pm |
Recap/Evaluation |
Schwartz
Klassen
Palmer |
| |
5:00pm |
Free Time |
-------- |
| |
7:00pm |
Dinner |
Schwartz
Klassen |
| |
|
|
|
|
Friday |
8:00am |
Review/Breakfast |
Schwartz
Klassen |
| |
9:00am |
Lecture: Flow Cytometry
Lab: FACS Analysis I
Characterization |
Schwartz
Klassen
|
| |
12:00pm |
Lunch/Journal Club |
Schwartz
Klassen
|
| |
1:30pm |
Lab: FACS Analysis II
Characterization |
Schwartz
Klassen
|
| |
4:00pm |
Recap/Evaluation |
Schwartz
Klassen |
| |
5:00pm |
Free Time |
-------- |
| |
7:00pm |
Dinner |
Schwartz
Klassen |
| |
|
|
|
|
Saturday |
8:00am |
Review/Breakfast |
Schwartz
Klassen |
| |
9:00am |
Lab: Immunocytochemistry I
Characterization |
Schwartz
Klassen
|
| |
12:00pm |
Lunch/Journal Club |
Schwartz
Klassen
|
| |
1:30pm |
Free Time |
-------- |
| |
4:00pm |
Free Time |
-------- |
| |
5:00pm |
Free Time |
-------- |
| |
7:00pm |
Free Time |
-------- |
| |
|
|
|
|
Sunday |
8:00am |
Free Time |
-------- |
| |
9:00am |
Free Time |
-------- |
| |
12:00pm |
Lunch |
Schwartz
Klassen
|
| |
1:30pm |
Lab: Immunocytochemistry II
Characterization |
Schwartz
Klassen |
| |
4:00pm |
Recap/Evaluation |
Schwartz
Klassen |
| |
5:00pm |
Free Time |
-------- |
| |
7:00pm |
Dinner
Introduction of Dr. Carpenter |
Schwartz
Klassen
Carpenter
Loring |
| |
|
|
|
|
Monday |
8:00am |
Review/Breakfast |
Schwartz
Klassen
Carpenter
Loring |
| |
9:00am |
Lecture: Expansion of hESCs
Feeder Free hESCs
Lab: Expansion of hESCs
Derivation of ES cells and neural stem cells & Maintenance of human ES cells
|
Schwartz
Klassen
Carpenter
Loring |
| |
12:00pm |
Lunch/Journal Club |
Schwartz
Klassen
Carpenter |
| |
1:30pm |
Lecture: Neural Differentiation of hESCs
Lab: Neural Differentiation of hESCs
Differentiation
|
Schwartz
Klassen
Carpenter
Loring |
| |
4:00pm |
Recap/Evaluation |
Schwartz
Klassen
Carpenter |
| |
5:00pm |
Free Time |
-------- |
| |
7:00pm |
Dinner
Introduction of Dr. Duncan |
Schwartz
Klassen
Duncan |
| |
|
|
|
|
Tuesday |
8:00am |
Review/Breakfast |
Schwartz
Klassen
Duncan |
| |
9:00am |
Lecture: Purification and Differentiation of Oligo Precursors
Differentiation |
Schwartz
Klassen
Duncan |
| |
12:00pm |
Lunch/Journal Club |
Schwartz
Klassen
Duncan |
| |
1:30pm |
Lab: Purification and Differentiation of Oligo Precursors
Differentiation |
Schwartz
Klassen
Duncan |
| |
4:00pm |
Recap/Evaluation |
Schwartz
Klassen |
| |
5:00pm |
Free Time |
-------- |
| |
7:00pm |
Dinner |
Schwartz
Klassen
Duncan
Loring |
| |
|
|
|
|
Wednesday |
8:00am |
Review/Breakfast |
Schwartz
Klassen
Duncan
Loring |
| |
9:00am |
Lecture: IVF and hESCs
Harvest of hNSCs
Separation Techniques
Derivation of ES cells and neural stem cells |
Schwartz
Klassen
Loring |
| |
12:00pm |
Lunch/Journal Club |
Schwartz
Klassen
Loring |
| |
1:30pm |
Lab: Separation Techniques
Derivation of ES cells and neural stem cells |
Schwartz
Klassen
Loring |
| |
4:00pm |
Recap/Evaluation |
Schwartz
Klassen
Loring |
| |
5:00pm |
Free Time |
-------- |
| |
7:00pm |
Dinner |
Schwartz
Klassen
Chiu
Loring |
| |
|
|
|
|
Thursday |
8:00am |
Review/Breakfast |
Schwartz
Klassen
Chiu
Loring |
| |
9:00am |
Lecture: PCR/Karyotyping
Lab: PCR/Karyotyping
Characterization |
Schwartz
Klassen
Chiu
Loring |
| |
12:00pm |
Lunch/Journal Club |
Schwartz
Klassen
Chiu
Loring |
| |
1:30pm |
Lecture: NIH Regulations
Lab: Executing MTAs
NIH funding and policies |
Schwartz
Klassen
Chiu
Loring |
| |
4:00pm |
Recap/Evaluation
|
Schwartz
Klassen
Chiu
Loring |
| |
5:00pm |
Free Time |
-------- |
| |
7:00pm |
Dinner |
Schwartz
Klassen
Chiu
Loring |
| |
|
|
|
|
Friday |
Departure |
|
|
|
|
